Our group is interested in elucidating how the mammalian (epi)genome is maintained throughout cell divisions and how the epigenetic information is translated into spatial chromatin structure and activity during differentiation, reprogramming and disease.
We make use of a variety of biochemical, molecular and cell biological methods with particular emphasis on advanced live-cell and super-resolution fluorescence microscopy.
In the course of our work, we develop new approaches to deliver macromolecules to living cells and establish tools including nanobodies to visualize subcellular structures and cell cycle progression in real time.
<
🧬 New preprint! When replication forks stall, the replisome doesn't just wait. Live-cell imaging of endogenously GFP-tagged factors shows polymerases pile up even with DNA synthesis blocked — because dormant origins fire right next to stalled forks.
doi.org/10.64898/202...
www.cardoso-lab.org
